| 產品編號 | bsm-52647R |
| 英文名稱 | Tissue factor Recombinant Rabbit mAb |
| 中文名稱 | 組織因子（CD142）兔單克隆抗體 |
| 別 名 | CD 142; CD-142; CD142; CD142 antigen; Coagulation factor III(thromboplastin tissue factor); Coagulation factor III; F3; TF; TFA; Thromboplastin; Tissue factor; TF_HUMAN. |
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Specific References (1) | bsm-52647R has been referenced in 1 publications.
[IF=5.118] Masahiro Terasawa. et al. Anti-Inflammatory Activity of Orally Administered Monostroma nitidum Rhamnan Sulfate against Lipopolysaccharide-Induced Damage to Mouse Organs and Vascular Endothelium. Mar Drugs. 2022 Feb;20(2):121 WB ; Mouse.
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| 研究領域 | 心血管 細胞生物 免疫學 生長因子和激素 細胞表面分子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 6A4 |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 35 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Tissue factor: 1-100/295 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010] Function: Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. Subunit: Interacts with HSPE; the interaction, inhibited by heparin, promotes the generation of activated factor X and activates coagulation in the presence of activated factor VII. Subcellular Location: Isoform 1: Membrane; Single-pass type I membrane protein. Isoform 2: Secreted. Tissue Specificity: Lung, placenta and pancreas. Similarity: Belongs to the tissue factor family. SWISS: P13726 Gene ID: 2152 Database links: Entrez Gene: 2152 Human Entrez Gene: 14066 Mouse Omim: 134390 Human SwissProt: P13726 Human SwissProt: P20352 Mouse Unigene: 62192 Human Unigene: 273188 Mouse Unigene: 9980 Rat |
| 產品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with Tissue factor monoclonal antibody, unconjugated (bsm-52647R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
