| 產品編號 | bsm-33193M |
| 英文名稱 | Cytochrome C Mouse mAb |
| 中文名稱 | 細胞色素C單克隆抗體 |
| 別 名 | CytC; CYC; CYCS; Cytochrome c somatic; HCS; CYC_HUMAN; Cytochrome c; MSA06; THC4. |
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Specific References (4) | bsm-33193M has been referenced in 4 publications.
[IF=4.927] Xiao-Jiao Chen. et al. Extracts of Knoxia roxburghii (Spreng.) M. A. Rau Induce Apoptosis in Human MCF-7 Breast Cancer Cells via Mitochondrial Pathways. MOLECULES. 2022 Jan;27(19):6435 WB ; Human.
[IF=3.708] Xuena Zhang. et al. Luteoloside Prevents Sevoflurane-induced Cognitive Dysfunction in Aged Rats via Maintaining Mitochondrial Function and Dynamics in Hippocampal Neurons. NEUROSCIENCE. 2023 Feb;: WB ; Rat.
[IF=2.57] Ding, Wensen, et al. "Increased expression of HERPUD1 involves in neuronal apoptosis after intracerebral hemorrhage." Brain Research Bulletin 128 (2017): 40-47. WB ; Rat.
[IF=0.603] Zhou BZ et al. Protective effect of cyclosporine A in the treatment of severe hydronephrosis in a rabbit renal pelvic perfusion model. Turk J Med Sci. 2019 Oct 24;49(5):1590-1598. WB ; Rabbit.
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| 研究領域 | 腫瘤 心血管 細胞生物 神經生物學 細胞凋亡 線粒體 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 6B10 |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 12 kDa |
| 檢測分子量 | 14.4 |
| 細胞定位 | 細胞漿 線粒體 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human Cytochrome C Protein |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in the process of oxidative phosphorylation and production of cellular ATP. An increasing amount of interest has been directed toward the role which cytocrome C has been demonstrated to play in apoptotic processes. Following exposure to apoptotic stimuli, cytochrome C is rapidly released from the mitochondria into the cytosol, an event which may be required for the completion of apoptosis in some systems. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis. Function: Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases. Subcellular Location: Mitochondrion intermembrane space. Note=Loosely associated with the inner membrane. Post-translational modifications: Binds 1 heme group per subunit. Phosphorylation at Tyr-49 and Tyr-98 both reduce by half the turnover in the reaction with cytochrome c oxidase, down-regulating mitochondrial respiration. DISEASE: Defects in CYCS are the cause of thrombocytopenia type 4 (THC4) [MIM:612004]; also known as autosomal dominant thrombocytopenia type 4. Thrombocytopenia is the presence of relatively few platelets in blood. THC4 is a non-syndromic form of thrombocytopenia. Clinical manifestations of thrombocytopenia are absent or mild. THC4 may be caused by dysregulated platelet formation. Similarity: Belongs to the cytochrome c family. SWISS: P99999 Gene ID: 54205 Database links: Entrez Gene: 54205 Human Entrez Gene: 13063 Mouse Omim: 123970 Human SwissProt: P99999 Human SwissProt: P62897 Mouse Unigene: 437060 Human 細胞色素C（cytC）是一種電子傳遞鏈蛋白為線粒體呼吸鏈必須的成份之一。在哺乳動物細胞中，如此高度保守性蛋白常分布在線粒體內膜。 新近研究證明細胞漿中細胞色素C為激活細胞調亡所必需的因子。在調亡的過程中，細胞色素C從線粒體膜被易位到細胞漿，由細胞色素C激活Caspase-3（CPP32）。 細胞色素C的易位可被過量表達的Bcl-2阻斷。細胞色素B與細胞色素C1和Rieske蛋白相結合而形成復合物III（也稱細胞色素B-C1復合物）參與細胞呼吸鏈。該蛋白動物種屬間同源性較高；如 ：豬、犬、牛、雞、豚鼠等。 |
| 產品圖片 |
Sample:
Lane 1: Mouse Heart tissue lysates
Lane 2: Mouse Muscle tissue lysates
Lane 3: Mouse Cerebrum tissue lysates
Lane 4: Rat Muscle tissue lysates
Lane 5: Human Jurkat cell lysates
Lane 6: Human HL-60 cell lysates
Lane 7: Human U251 cell lysates
Primary: Anti-Cytochrome C (bsm-33193M) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
Sample:
Hcclm3(Human) Cell Lysate at 30 ug
Primary: Anti-Cytochrome C (bsm-33193M) at 1/100000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 14 kD
Observed band size: 14 kD
Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytochrome C) Monoclonal Antibody, Unconjugated (bsm-33193M) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytochrome C) Monoclonal Antibody, Unconjugated (ascites of bsm-33193M 6B10) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.
2661號盒-20230303-LLL_20.0x-3-C.jpg)
Paraformaldehyde-fixed, paraffin embedded (human Prostate Tumor); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytochrome C) Monoclonal Antibody, Unconjugated (bsm-33193M) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
2661號盒-20230303-LLL_20.0x-5-C.jpg)
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytochrome C) Monoclonal Antibody, Unconjugated (bsm-33193M) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytochrome C) Monoclonal Antibody, Unconjugated (bsm-33193M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytochrome C) Monoclonal Antibody, Unconjugated (bsm-33193M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytochrome C) Monoclonal Antibody, Unconjugated (bsm-33193M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytochrome C) Monoclonal Antibody, Unconjugated (bsm-33193M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytochrome C) Monoclonal Antibody, Unconjugated (bsm-33193M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytochrome C) Monoclonal Antibody, Unconjugated (bsm-33193M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Cytochrome C) monoclonal Antibody, Unconjugated (bsm-33193M) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
